02. SP8 Liachroic confocal

The Liachroic SP8 is a typical confocal laser scanning microscope that is open to all FNWI-staff, but has been principally purchased for and by the Storkebaum group at DCN whose members can use advance priority-booking for 4 days a week from Tuesdays-Fridays. The system is freely bookable on Weekends and Mondays. Tuesdays-Fridays can still be booked in a last-minute manner, whenever same-day booking time is available. It can be used for wide-field and confocal fluorescence imaging, as well as bright field imaging. 

Specifications:

• Inverted DMi8 CS motorized microscope
• 2000 FOV scanner SP8
• Light source EL 6000

• Filter Cubes:
• DAPI LP (ex: 380-400, em: 425-infinite nm)
• GFP (ex: 450-490, em: 500-550)
• Y3 (ex: 533-558, em: 570-640)
• Y5 (ex: 590-650, em: 662-738)

• Obj. HC PL APO 10x/0.40 CS2  (FWD = 2.56 mm)
• Obj. HC PL FLUOTAR L 20x/0.40 (7.5-6.2 mm)
• Obj. HC PL APO 20x/0.75 CS2  (0.66 mm)
• Obj. HC PL FLUOTAR L 40x/0.60  (3.3-1.9 mm)
• Obj. HC PL APO 40x/1.30 Oil CS2  (0.24 mm)
• Obj. HC PL APO 63x/1.40 OIL CS2  (0.14 mm)

• 1 x PMT Detector
• 2 x HyD SP GaAsP Detector (sensitive)
• Brightfield detector for DMI

• Laser 405 nm DMOD Compact
• Laser Blue 488 nm
• Laser Green 552 nm
• Laser Red 638 nm

• Leica DFC7000 GT monochrome camera
• LAS X SP8 Control Software with Navigator Module