7. DMIRE2 inverted

The Leica DMIRE2 microscope is an inverted microcope equipped for bright field and fluorescence Wide-field imaging. A Photometrics CoolSnap K4 camera is attached. The camera, the filters turret, the zDrive, the XY stage and the transmitted light can be controlled by the uManager software.

Objectives: (Type, medium, Mag, NA, WD [mm], Max FOV [mm], catalog #)

• _{- HCPL fluotar Dry10x / 0.30 NA; 11.0 mm, maximum field of view 1.500 mm x 1.500 mm, 15506505}
• _{- HC PL apo CS 20x / 0.70 D, also for DIC; 0.59 mm, maximum field of view 750 µm x 750 µm, 15506513}
• _{- HCX apo Long working distance, Ultraviolet (VI) Water immersion 40x / 0.80 DIC; 3.3 mm,} 
• _{maximum field of view 375 µm x 375 µm, 15506155}
• _{HCX PL apo CS 63x / 1.30 Glycerol immersion 21 C; 0.28 mm, maximum field of view 238.1 µm x 238.1 µm, 15506194}
• _{- HCX Pl apo 63x / 1.40-0.60 (oil immersion with diaphragm) lambda blue-GFP; 0.10 mm,}
• _{maximum field of view 238.1 µm x 238.1 µm, 15506192}
•  

Fluorescence 
Filter cube positions:

• A for UV excitation BP340-380, DM 400, LP 425
• N2.1 for green excitation BP 515-560, DM 580, LP 590
• I3 for blue excitation BP 450-490, DM 510, LP 515
• Scan for empty = transmission position
• BP = band pass, DM = Dichroic mirror, LP = Long pass

Light source; HBO mercury lamp  (NOTICE: THE LAMPS SHOULD BE ALLOWED TO COOL DOWN BEFORE NEXT USE; RAPID OFF AND ON SWITCHING WILL HARM THE LAMP THAT MAY EVEN EXPLODE!)

Transmitted brightfield and fluorescence microscopy. Z-stacks, tile scans and time lapse acquisition.